Aseptic Technique And Culturing Microbes Coursework Example

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The microbiology lab report
Introduction
The aseptic method is a significant and necessary laboratory experience which is very necessary for the field of microbiology. Most of the Microbiologists prefer using the techniques as it is applicable for most of their procedures such as inoculating media, transferring and isolation of pure cultures as well as performing the microbiological tests. Additionally, it helps in eliminating of contamination of the specimen used in the microbial experiments. Materials and the methods
Voges-ProskauerUse two MR-VP broth tubes where one is labeled one E.coli and the other one S. epidermidis. Then inoculate the two test tubes with the appropriate culture. They are placed in the incubator at 37 degrees C for one day. After small incubation amount of each culture is put in a test-tube where one adds 6-8 drops of methyl orange (Li et al. 38). For the remaining part of the culture, one adds 12 drops of Barrett’s reagent. Then one observes the tubes for the gradual formation of pink to red color.
Motility testing
Incubate E.coli and S. epidermidis for one day. Then make A Wet mold of the two cultures. Two motility medium are labeled E.coli and S. epidermidis respectively where the inoculated cultures are put (Bredenoord et al. 786). The two test tube is then incubated 370 for one day from which one record the results. Carbohydrate fermentation
Three red phenol tubes labeled E.coli and another three labeled S. epidermidis.

The Durham tubes are then put upside down on the six phenol tubes. Then inoculate the phenol tubes. They are then placed in incubator 370 for about one day (Phipps 569). Then observe the color of the phenol tubes.
Results
Biochemical testing and microbial identification Escherichia coli Staphylococcus epidermidis Saccharomyces cerevisiae
methyl red
Its color turned red. Thus it is +ve for MR It has a yellowish color thus it is -ve for MR. N/A
Voges-ProskauerIt is VP -ve since it is yellowish.
It is +ve for VP as it has a red color. N/A
Catalase LabPaq experiment  Has –ve catalase since it has no bubbles Has +ve catalyze since it showed bubbles. N/A
carbohydrate fermentation N/A The tubes for glucose and fructose tubes was +ve for acid as they were yellow The acid tube was +ve for acid while the glucose tube was yellowish- orange
motility LabPaq experiment  It has positive motility Has negative motility N/A
Discussion
The Voges-Proskauer determines the MO ability to oxidase the floccose into neutral end product basing on the enzymatic pathways which are used in the bacteria differentiate Enterobacter as well as Klebsiella from the E.coli. On the other hand, the positive results in carbohydrate fermentation and motility LabPaq experiment indicates that specific organism can produce enough acidity end product which is responsible for lower the pH below 5.0.
Work cited
Bredenoord, Albert J., and André J. Smout. “Esophageal motility testing: impedance-based transit measurement and high-resolution manometry.” Gastroenterology Clinics 37.4 (2008): 775-791.
Li, Jiqiu, Beiping Tan, and Kangsen Mai. “Dietary probiotic Bacillus OJ and isomaltooligosaccharides influence the intestine microbial populations, immune responses and resistance to white spot syndrome virus in shrimp (Litopenaeus vannamei).” Aquaculture 291.1-2 (2009): 35-40.
Phipps, Linda R. “Creating and teaching a web-based, university-level introductory chemistry course that incorporates laboratory exercises and active learning pedagogies.” Journal of Chemical Education 90.5 (2013): 568-573.