Ubiquity in microorganisms Coursework Example

Ubiquity in microorganisms
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Abstract
Microorganisms are microscopic organisms only visualized by using a microscope. Every known habitat to man contains microorganisms. We normally have prokaryotic microorganisms which are bacteria. There are also algae which are sometimes referred to as eukaryotic, and protozoa/fungi. Viruses though acellular infect cells severely. Bacteria live in colonies despite being unicellular and sometimes in multicellular forms. The diversity and ubiquity of microorganisms can be observed deeply to come up with various conclusions. Many bacteria inhabiting a human environment are normally harmless and they coexist with life. Some bacteria are existing in symbiotic relationships with their hosts. Sometimes, some bacteria express opportunistic behaviors to become pathogenic. Such bacteria compete with the cells of the host for foods and nutrients and hence harming life. When such bacteria are showing such ideas, they occupy the wrong place at the wrong place in the host’s body and causing diseases and complications.
Keywords: microorganisms, microscopic, acellular.
The experiment below has sued asceptic techniques to show how to handle bacteria, (Coates, et.al, 2002).
Methods
1. Wear protective kits for your hands (gloves).
2. Keep laboratory cultures and items far from children’s reach.
3. Label all containers containing the microbes well.
4. Put the colonies in the broth as a suspension.
5. Compare the cell’s turbidity with the one in figure 15.

NB: make sure the metal inoculation is sterilized well.
6. Carefully extinguish the flame.
7. Use a pipette to remove the broth’s contents aseptically.
8. Label the dilutions in the vial accordingly as M1, M2, M3, M4, and B1, B2, B3, B4.
9. Add drops of bacteria into the dilutions, 3 drops into each.
10. Add up to three drops of bacterial culture into the vial C.
11. Return the remaining culture into the stock vial.
12. Use the bleach bucket to store the empty pipette.
13. Cap each vial and store them into the placed.
14. Capture a photo of each of the mouthwash dilution vials and name it.
15. Take a photo of each of the bleach dilution together with the control vial.
16. Incubate the tubes at room temperature for one day. Observe and record down any processes taking place.
17. Incubate the vials for another two days while recording any observations.
18. Stop the process of incubation once the there is a distinct turbidity in the control vial.
19. Make an observation of the turbidities of each content while noting that the ones lacking turbidity are the MIC.
20. Capture a photogram of the mouthwash contents.
21. Label the MIC vial mouthwash
22. Take another photograph of the bleach vials together with the control vial.
23. The MIC vial in the dilution series should also be labeled.
After the processes, make sure everything is bleached for one hour with unused bleach.
Use 10 bleach to wipe down the workplace.
Clean and return the tools back where they should be stored.
Questions
It is necessary that during the process of incubation all the plates are inverted to prevent condensation from occurring on the surface medium. The antiseptic technique was the most effective in performing inhibition since everything was sterilized.
From tube 2 to 4, the dilution of the MIC was as 0.5/5*11= 1.1.
The purpose of the control tube in the experiment is to make comparisons with the effects of storing something in and out of the experiment.
Conclusion
The experiment was very successful in that everything was observed and the reactions of the microorganism with bleaching agents shown, (Coates, et.al, 2002). The antiseptic technique showed more inhibition of the microorganisms’ additions. The results compare well to the real world in that they demonstrate fully how microorganisms form and live. The experiment demonstrates how it is possible to counter the population of microorganisms and particularly the opportunistic microorganisms that can damage a host. The experimental results substantiate the theory of how to counter microorganisms through sterilization and antiseptic. They grow in wet places and reproduce fast in large numbers. The results were not very consistent with the original thinking since there were various errors and challenges. I was forced to revise my original thinking due to the various challenges. The errors observed may be due to environmental factors and also due to poor observations. The addition of the drops may be less.
References
Coates, J. D., Cole, K. A., Chakraborty, R., O’Connor, S. M., & Achenbach, L. A. (2002). Diversity and ubiquity of bacteria capable of utilizing humic substances as electron donors for anaerobic respiration. Applied and environmental microbiology, 68(5), 2445-2452.